2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

Related Products

PDF 1.61 MB

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-N4321
    Glucotropaeolin potassium
    		99.63%
    Glucotropaeolin potassium (Benzylglucosinolate potassium) , a glucosinolate contained in cruciferous vegetables, causes a moderate decrease in spontaneous DNA damage in animals.
    Glucotropaeolin potassium
  • HY-137843
    NSC 80467
    		Inhibitor 98.04%
    NSC 80467, a DNA damaging agent, selectively inhibits survivin. NSC 80467 preferentially inhibits DNA synthesis and results in induction of γH2AX and pKAP1, two markers of DNA damage.
    NSC 80467
  • HY-100624
    Ryuvidine
    		Inhibitor 99.40%
    Ryuvidine is a potent inhibitor of SET domain-containing protein 8(SETD8) with an IC50 of 0.5 µM and suppresses monomethylation of H4K20. Ryuvidine also inhibits CDK4 with an IC50 of 6.0 μM. Ryuvidine also inhibits KDM5A and blocks DNA synthesis. Ryuvidine has anticancer activity against tumors such as breast cancer. Ryuvidine improves arthritis.
    Ryuvidine
  • HY-160859
    WRN inhibitor 8
    		Inhibitor 98.07%
    WRN inhibitor 8 (Example 224) is a WRN helicase inhibitor with IC50 value of 48 nM. WRN inhibitor 8 can be used in cancer research.
    WRN inhibitor 8
  • HY-153601
    MC-VC-PAB-Cyclohexanediamine-Thailanstatin A
    MC-VC-PAB-Cyclohexanediamine-Thailanstatin A is a Spliceostatin (HY-16466) analog, and a drug-linker conjugates for ADC, consisting of ADC Cytotoxin Thailanstatin A (HY-129589) and cleavable ADC linker (MC-vc-PAB). MC-VC-PAB-Cyclohexanediamine-Thailanstatin A can be used for ADC synthesis.
    MC-VC-PAB-Cyclohexanediamine-Thailanstatin A
  • HY-N0157C
    Orotic acid potassium
    		99.51%
    Orotic acid potassium (Vitamin B13 potassium) is a precursor of pyrimidine bases and is involved in the synthesis of DNA and RNA. Orotic acid potassium stimulates the growth of animals, plants and microorganisms, participates in carbohydrate metabolism, and is necessary for the growth and life activities of organisms. Orotic acid potassium is a measurement indicator in routine newborn screening for urea cycle abnormalities. Orotic acid potassium can cause hepatic steatosis and hepatomegaly in rats.
    Orotic acid potassium
  • HY-163942
    GSK_WRN4
    		Inhibitor 98.85%
    GSK_WRN4 is a WRN helicase inhibitor (pIC50=7.6) with anticancer activity. GSK_WRN4 selectively inhibits the growth of MSI tumor cells in vitro and in vivo by inducing DNA double-strand breaks, particularly at expanded TA repeats and DNA damage regions.
    GSK_WRN4
  • HY-E70086
    Taq DNA polymerase
    Taq DNA polymerase is a thermostable DNA polymerase that can be used in PCR.
    Taq DNA polymerase
  • HY-135780A
    3'-Deoxyuridine-5'-triphosphate trisodium
    		Inhibitor 99.69%
    3'-Deoxyuridine-5'-triphosphate trisodium (3'-dUTP trisodium) is a nucleotide analogue that inhibits DNA-dependent RNA polymerases I and II. 3'-Deoxyuridine-5'-triphosphate trisodium strongly and competitively inhibits the incorporations of UTP into RNA with a Ki value of 2.0 μM.
    3'-Deoxyuridine-5'-triphosphate trisodium
  • HY-133157
    FAICAR
    FAICAR (5-Formamidoimidazole-4-carboxamide ribotide) is a purine nucleotide.
    FAICAR
  • HY-W093086
    DMT-2'-F-Bz-dA
    		99.58%
    DMT-2'-F-Bz-dA can be used in the synthesis of nucleotides and nucleic acids.
    DMT-2'-F-Bz-dA
  • HY-20140
    5'-O-DMT-dT
    		99.09%
    5'-O-DMT-dT (5'-O-(4,4'-Dimethoxytrityl)thymidine) is a nucleoside derivative which can be used in the preparation of oligonucleotides.
    5'-O-DMT-dT
  • HY-17026R
    Gemcitabine (Standard)
    		Inhibitor 99.99%
    Gemcitabine (Standard) is the analytical standard of Gemcitabine. This product is intended for research and analytical applications. Gemcitabine (LY 188011) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine inhibits DNA synthesis and repair, resulting in autophagyand apoptosis.
    Gemcitabine (Standard)
  • HY-16637R
    Folic acid (Standard)
    		98.39%
    Folic acid (Standard) is the analytical standard of Folic acid. This product is intended for research and analytical applications. Folic acid (Vitamin B9) is a orally active essential nutrient from the B complex group of vitamins. Folic acid shows antidepressant-like effect. Folic acid sodium reduces the risk of neonatal neural tube defects. Folic acid can be used to the research of megaloblastic and macrocytic anemias due to folic deficiency.
    Folic acid (Standard)
  • HY-145975A
    m7Gpppm6AmpG (ammonium) solution (100mM)
    		98.84%
    m7Gpppm6AmpG ammonium solution (100mM) is a trinucleotide mRNA 5’ cap analogs. m7Gpppm6AmpG ammonium can be used for RNA synthesis in vitro.
    m7Gpppm6AmpG (ammonium) solution (100mM)
  • HY-B0417A
    Lincomycin hydrochloride
    		Inhibitor 99.29%
    Lincomycin hydrochloride is the salt form of the antibiotic lincomycin. Lincomycin can affect the synthesis of protein, RNA and DNA in the mycelium of the lincomycin-producing organism Act. Roseolus. Lincomycin inhibits protein synthesis in gram-positive and - to a lower degree - also in gram-negative bacteria. Lincomycin binds to the 50S subunits of bacterial ribosomes and act on aminoacyl-tRNA binding and the transpeptidation reaction.
    Lincomycin hydrochloride
  • HY-145555
    Bersiporocin
    		Inhibitor 98.99%
    Bersiporocin is a prolyl-tRNA synthetase inhibitor. Bersiporocin has an IC50 of ≤100 nM for phosphoribosylpyrophosphate synthetase (PRS). Bersiporocin can be used for the research of antifibrotic.
    Bersiporocin
  • HY-122903A
    (-)-TK216
    		Inhibitor 99.45%
    (-)-TK216 is an enantiomer of TK216 (HY-122903). TK216 is an orally active and potent E26 transformation specific (ETS) inhibitor. (-)-TK216 has anti-cancer activity.
    (-)-TK216
  • HY-112081
    BAY-707
    		Inhibitor 99.64%
    BAY-707 is a substrate-competitive, highly potent and selective inhibitor of MTH1(NUDT1) with an IC50 of 2.3 nM. BAY-707 has a good pharmacokinetic (PK) profile to other MTH1 compounds and is well-tolerated in mice, but shows a clear lack of in vitro or in vivo anticancer efficacy.
    BAY-707
  • HY-144645
    SP-471P
    		Inhibitor 99.46%
    SP-471P is a potent dengue virus (DENV) protease inhibitor with EC50s of 5.9 μM, 1.4 μM, 5.1 μM and 1.7 μM for DENV1, DENV2, DENV3 and DENV4, respectively and CC50 value over 100 μM. SP-471P can reduce DENV viral RNA synthesis.
    SP-471P
Cat. No. Product Name / Synonyms Application Reactivity